Exploring Biochemical Tests in Bacteriology
Catalase Test Principle The enzyme catalase facilitates the decomposition of hydrogen peroxide into water and oxygen. Example Staphylococcus Catalase Positive Bubbles appear. Streptococcus Catalase Negative Bubbles not appear. Coagulase Test Principle Coagulase is an enzyme that turns a liquid called fibrinogen into a sticky substance called fibrin. It's used to check if bacteria can make blood clot." Example Coagulase Positive Staphylococcus aureus . Coagulase Negative Staphylococcus epidermidis. Oxidase Test Principle The oxidase test identifies the presence of cytochrome c oxidase enzyme in bacteria. Or the oxidase test is to detect the presence of the enzyme cytochrome c oxidase in bacteria. This enzyme is involved in the electron transport chain of aerobic respiration and catalyses the oxidation of cytochrome c by molecular oxygen. Example Oxidase Positive Pseudomonas aeruginosa . Oxidase Negative E. coli . Urease Test Pr